Bacterial Cell Envelope Lysis and Hemotoxicity of Peptides Previously isolated From African Catfish, Clarias gariepinus

Background: The skin mucus layer of fish is endowed with biologics including, Antimicrobial peptides (AMPs) that offer a first line defence against pathogens. Such can either inhibit bacterial growth or completely kill the bacteria and hence are regarded as viable alternative to traditional antibiotics, in addressing ever-increasing incidences antimicrobial resistance. However, one major hurdles AMPs use their poor haemolytic profile. As result, thorough evaluation prospective AMPs’ cell membrane disruption hemolytic potentials early phases drug discovery critical. current study presented destruction well hemo-compatibility previously isolated from African catfish, Clarias gariepinus. Methods: A peptide C. gariepinus were profiled using 15% Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). electrical conductivity alkaline phosphatase assays utilised measure envelope lysis activity classical mode action peptides. Afterwards, fresh Rabbit blood cells then for vitro assay. Results: found be about 5 kDa molecular weight with, ability damage causing significant leakage periplasmic enzyme cytoplasmic electrolytes. Even at highest extract concentration 100 μg/mL, no hemolysis was observed on rabbit [3.63%;P>.05], signifying safety normal mammalian cells. Conclusion: findings this pointed out potentially safe source leads; however, further studies still required search possibly maximum dose host but effective infecting bacteria.